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The effects of different concentrations of three types of cosolvents on the recovery and stability of a type A botulinum neurotoxin produced by Lactobacillus jensenii were investigated. Results of a trypsin stability assay indicated that denaturation and degradation of the neurotoxin occurred at a concentration of the cosolvent-to-water ratio of 1:4. The ability of the neurotoxin to intoxicate mice was found to be significantly affected at a 1:2 cosolvent-to-water ratio. This ratio was associated with a slight, but not significant, increase in the median lethal dose. The presence of water decreased the time-dependent inactivation of the toxin in saline solution at 37 degrees C. On the other hand, the presence of 0.05 M sodium chloride, 0.1 M phosphate, and 0.1 M ammonium bicarbonate pH 7.2 in the saline solution protected the botulinum neurotoxin from time-dependent inactivation at 37 degrees C. Although reduction in the inactivation rate was seen during storage in a refrigerator and on ice, the neurotoxin remained biologically active throughout 7 days of storage. These findings suggest the feasibility of an effective method for the cold storage of botulinum neurotoxin.After announcing this week that it will release a new remake of the original Zelda for the Wii U, Nintendo has now released a list of the games that will
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